Get ready for epitranscriptomics:
bit.ly/Kp99bL (Biotechniques newsy intro)
bit.ly/Kp9XNL (Cell paper)
Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 32 UTRs and near Stop Codons
Meyer, Kate D.; Saletore, Yogesh; Zumbo, Paul; Elemento, Olivier; Mason, Christopher E.; Jaffrey, Samie R.
Cell doi:10.1016/j.cell.2012.05.003
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mRNA Methylation Enriched in 3' UTRs near Stop Codons
Posted: May 17th, 2012, 8:13am EDT
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smears in Western blots? (are the proteins degraded) :((
Posted: May 17th, 2012, 3:44am EDT
I'm wondering how to properly store bacterial cell lysates? Do you add protease inhibitors before putting them at -20C or lower temperatures? When will the samples last?
Because, I've done Western blots of the cell lysates. The first time I did it, the bands looked neat and I still could quantify the bands. But when I tried to do the blots for the 3rd and 4th time, I only saw smears.. What happened??? -
Picrosirius red solution
Posted: May 17th, 2012, 1:04am EDT
I am trying to make up fresh picrosirius red solution as the solutions currently in the lab are rather old.
The protocol I followed says add 500mg sirius red F3B to 500ml saturated aqueous picric acid. I bought in the saturated picric acid solution and added the dye, however it is not going into solution (presumably because it is already a saturated solution). Apparently this hasn't been a problem before.
Does anyone have any suggestions please?
Thanks.
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lift off ADSC
Posted: May 16th, 2012, 4:49am EDT
I have isolated the Asipose Derived Stem Cells and differentiated them to the osteogenic differentiation with induction medium.The point is that I saw the lift off of the cells from the plate surface.
Is there any suggestion what would be the possible reason? -
Characterization of Zebrafish Retinal Connexin Hemichannels
Posted: May 16th, 2012, 4:46am EDT
Sun Z, Risner ML, van Asselt JB, Zhang DQ, Kamermans M, McMahon DG. Physiological and Molecular Characterization of Connexin Hemichannels in Zebrafish Retinal Horizontal Cells. J Neurophysiol. 2012 Feb 22. [Epub ahead of print]
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Caspase 8-mediated Apoptosis, TRADD TRAF, fish virus
Posted: May 15th, 2012, 12:42pm EDT
He BL, Yuan JM, Yang LY, Xie JF, Weng SP, Yu XQ, He JG. The Viral TRAF Protein (ORF111L) from Infectious Spleen and Kidney Necrosis Virus Interacts with TRADD and Induces Caspase 8-mediated Apoptosis. PLoS ONE. 2012;7(5): e37001. doi:10.1371/journal.pone.0037001
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HP 4500 ICP-MS service specialist/technician
Posted: May 15th, 2012, 10:12am EDT
Dear All,
Could you please recommend me a good service technician for an HP4500 ICP-MS. Your input is greatly appreciated.
Viv. -
2-D gel problem urgent please help
Posted: May 15th, 2012, 5:00am EDT
hi guys please help me out i am working on plant proteomics and i am trying very hard to get good 2-d gels but all in vain. i dont know where the problem lies i have tried multiple extraction protocols and rehydration buffer compositions but nothing is working. i have tried different pH 7cm strips like 3- 10, 5-8, 4-7. complete 2-D instrumentation is from Bio-Rad. for the gel pic i have attached the sample was extracted from plant using phenol extraction method followed by ammonium acetate... -
Cell Culture Technician Wanted
Posted: May 15th, 2012, 5:00am EDT
Cell Culture Technician
The Stowers Institute has an opening for a Cell Culture Technician. This position will include weekend work.
Responsibilities include maintaining mammalian and insect cell lines; expanding cell lines to meet researchers requirements; cultivating cells on plates or in flasks, and in roller bottles and/or spinner flasks for larger-scale (10-100 liter) cultures; Participating in the scheduling and delivery of all cell lines as requested; preparing and maintaini... -
retroviral infection using platE cells
Posted: May 15th, 2012, 2:45am EDT
Hi all,
I am trying to intrduce my gene of interset by retroviral infection into MEFs. for this first I do transfection in platE cells and then collect the viral supernatant to infect MEFs. then i start the selection of infected MEFs. but the problem is, all my cells (MEFs) die during selection!!!!! I have tried everything till now, but nothing is working...
any suggestions will really welcome...
thanks in advance!