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mycobacteria growth
Posted: January 31st, 2012, 7:01pm EST
Can mycobacteria (H37RV) minus plasmids grow in kanamycin agar? -
His-Tag Recombinant Protein purification
Posted: January 31st, 2012, 8:30am EST
Hello,
I am purifying a six-His tagged 50kDa hnRNP K protein from E coli cells using IMAC with Cobalt TALON resin. I am trying to send the proteins for crystallography and it requires a good quality of sample. My problem is that smaller bands are visible on the gel and I don't know how to get rid of them.
What might the problem and how can I fix it?
Can using a whole range of gradients (50, 60, 70, 80, 90, 100, 110mM, etc.), or adding imidazole to the wash buffer improve... -
PE Elan 6100 having low Cu recoveries for 6020 Interference Check
Posted: January 31st, 2012, 7:51am EST
Hi all,
I would like some advice on what could be the issue when an Elan 6100 is consistently failing the Interference check B for method 6020 for Copper. I am new on this machine, and with this method, and I am trying to find out where I should check. My trainer tells me (swears up down, left and right) that it is the machine and not the solution, the question is where in the machine?
The calibration curve is good, every other analyte in the interference check solution is p... -
Low MW peptide detection on SDS PAGE
Posted: January 30th, 2012, 2:42pm EST
Hi everybody, I have problem to detect 2KDa oligopetide on SDS PAGE. I made 17% Tris-Glycine gel and stained with CBB but didn't work as usual. Anybody does have a protocol for low MW peptide? I' going to do western blot on this SDS PAGE. I heard about Tricine gel but don't have all stuff and no money to buy them. Thanks in advance -
weird sine-wave oscillations?
Posted: January 30th, 2012, 10:07am EST
Hello,
I was recording some ligand-gated ionic currents with a voltage step protocol, and I got puzzled, why are all these sine wave-like oscillations appearing at the negative potentials that seemingly do not carry any channel currents? Are they normal, or are they just instrumental noise? coz I think I definitely have some channel-mediated current at some more positive potentials on the left one.
Just wonder if I can get any help?
Thanks
And -
Theoretical Combustion Chemistry
Posted: January 30th, 2012, 8:10am EST
KAUST (King Abdullah University of Science and Technology)
NATURE AND SCOPE OF JOB
Theoretical Combustion Chemistry
The KAUST Clean Combustion Research Center (CCRC) is seeking a candidate for a postdoctoral research position to work with leading scientists in the development of detailed chemical kinetic models for transportation fuels. The candidate will use software tools to estimate thermodynamic and transport properties, develop reaction pat... -
Technician in Institute of Genetic Medicine
Posted: January 30th, 2012, 6:30am EST
Ref A1084T
Faculty/Services Medical Sciences
Department Institute of Genetic Medicine
Job Type &... -
Technician
Posted: January 30th, 2012, 6:28am EST
Ref A1045T (ICM)
Faculty/Services Medical Sciences
Department Institute of Cellular Medicine
Job Type Technical and IT
Hours of Work &n... -
Senior Research Associate
Posted: January 30th, 2012, 6:26am EST
Ref A1083R (IAH)
Faculty/Services Medical Sciences
Department Institute for Ageing & Health
Job Type Research
Hours of Work &nb... -
Research Technician
Posted: January 30th, 2012, 6:23am EST
Ref A1063T (ICM)
Faculty/Services Medical Sciences
Department Institute of Cellular Medicine
Job Type ... -
Research Associate to Investigate the Origins of Eukaryotes using Statistic
Posted: January 30th, 2012, 6:21am EST
Ref A1043R (ICAMB)
Faculty/Services Medical Sciences
Department Institute for Cell & Molecular Biosciences
Job Type &n... -
Research Associate - Cardiovascular Development and Regeneration
Posted: January 30th, 2012, 6:18am EST
Ref A1053R (IGM)
Faculty/Services Medical Sciences
Department Institute of Genetic Medicine
Job Type Research
Hours of Work... -
Research Associate in Northern Institute for Cancer Research
Posted: January 30th, 2012, 6:15am EST
Ref A1090R (NICR)
Faculty/Services Medical Sciences
Department Northern Institute for Cancer Research
Job Type Research
... -
Research Associate in Institute for Cell Molecular Biosciences
Posted: January 30th, 2012, 6:12am EST
Ref A1087R
Faculty/Services Medical Sciences
Department Institute for Cell & Molecular Biosciences
Job Type &nbs... -
Research Associate in Institute of Cellular Medicine
Posted: January 30th, 2012, 6:10am EST
Ref A1065R (ICM)
Faculty/Services Medical Sciences
Department Institute of Cellular Medicine
Job Type &n... -
Research Associate
Posted: January 30th, 2012, 6:07am EST
Ref A1057R (IHS)
Faculty/Services Medical Sciences
Department Institute of Health & Society
Job Type... -
Research Assistant/Associate
Posted: January 30th, 2012, 6:04am EST
Ref A1064R (IHS)
Faculty/Services Medical Sciences
Department Institute of Health & Society
Job Type Research
Hours of Work &n... -
Lecturer in Cognitive Neuroscience
Posted: January 30th, 2012, 6:00am EST
Ref A1079A (IoN)
Faculty/Services Medical Sciences
Department Institute of Neuroscience
Job Type Academic (non-clinical)
Hours of Work &... -
Director of Learning, Teaching and Student Experience/Senior Lecturer
Posted: January 30th, 2012, 5:58am EST
Ref B467A (NUBS)
Faculty/Services Humanities, Arts & Social Sciences
Department Newcastle University Business School
Job Type Academic (non-clinica... -
Lecturer at Newcastle University
Posted: January 30th, 2012, 5:56am EST
Ref A1067A (IHS)
Faculty/Services Medical Sciences
Department Institute of Health & Society
Job Type Academic (non-clinical)
Hours of Work  ... -
Clinical Research Fellowships in Translational Medicine
Posted: January 30th, 2012, 5:53am EST
Ref A1041R (CD)
Faculty/Services Medical Sciences
Department Institute of Cellular Medicine
Job Type Clinical Academic/Research
Hours of Work ... -
ESRC-funded studentships in human experimental psychology
Posted: January 30th, 2012, 5:49am EST
ESRC funded studentships available in human experimental psychology
Possible Supervisors:
Professor Daniel Nettle, Dr Tom Smulders, Dr Gilbert Roberts, Dr Melissa Bateson and Dr Martin Tovee
Institute of Neuroscience, Newcastle University
Duration: 3 Years
Project description:
Projects in human experimental psychology, specifically:
Episodic Memory
Cooperative and normative behaviour
A... -
PhD position at Newcastle University
Posted: January 30th, 2012, 5:45am EST
Expression of autism susceptibility genes in the earliest stages of human cerebral cortex development
Supervisors:
Dr Gavin Clowry, Institute of Neuroscience http://www.ncl.ac.uk/ion/staff/profile/gavin.clowry
Professor Susan Lindsay, Institute of Genetic Medicine
http://www.ncl.ac.uk/ion/staff/profile/susan.lindsay
Sponsor:
Anatomical Society of Great Britain and Ireland
http://www.anatsoc.org.uk/
Duration of the award: 3 years from 1st October 20... -
Faculty Positions at King Abdullah University
Posted: January 26th, 2012, 5:44am EST
A Global University
King Abdullah University of Science and Technology (KAUST) is an international graduate-level research university dedicated to advancing science and technology through breakthrough bold and collaborative research.
KAUST was built to address scientific challenges of regional and global significance through interdisciplinary research. The university promotes a culture of cross-discipline collaboration and encourages faculty to break down the barriers between field... -
young scientist at GIBH
Posted: January 26th, 2012, 5:25am EST
The Institute is now seeking young scientists with great vigor and passion for the full time faculty
positions in any of the following disciplines:
South China Institute for Stem Cells and Regeneration Medicine
1) Preclinical Research of Stem Cells
2) Establishment of Non-human Primate Animal Models
Institute for Chemical Biology
3) Computer-Aided Drug Design
4) Molecular and Cell Biology
5) Medicinal Chemistry
6) Enzyme Catalytic Reaction
Center of Inf... -
Invites Applications for One Centre Director and Two Principal Investigator
Posted: January 26th, 2012, 5:20am EST
These positions are funded by “The Hundred Talents Program” and “The Thousand Talents Program”
The Kunming Primate Research Centre, established in 2005, is a non-corporatesubunit of the Kunming Institute of Zoology, Chinese Academy of Sciences. The centre houses multiple primate species, including Macaca mulatta, M. fascicularis, M. arctoides, M. assamensis, M. nemestrina, Rhinopithecus roxellana, the Northern Treeshrew Tupaia belangeri, and other species. The Cent... -
primate GLP safety assessment centre director position
Posted: January 26th, 2012, 5:18am EST
The Kunming Institute of Zoology,one of the 20 biological institutes under the Chinese Academy of Sciences, is also one of China's top class zoological research institutes.
The primate GLP safety assessment centre of Kunming Institute of Zoology is a key project of China National Development and Reform Commission It aims to provide primate drug safety evaluations which meet China Drug Administration and American FDA/GLP requirements for medical enterprises and scientific research institut... -
Scientific Manager -Analytical Developmen
Posted: January 26th, 2012, 5:12am EST
Job Category Medical/Healthcare/Bio-Tech
Location Bangalore
Experience 5.00 to 12.00 years
Education MSc, PHD
Skills
Qualifications: MPharm/M.Sc (5-8 years exp) or PhD (3-5 years exp) with Analytical (Bio)Chemistry background. PhD in Chemistry, Biochemistry, Biophysical Chemistry or Life Science Experience in industry a must. Should have thorough knowledge on analytical method using... -
Executive/Sr Executive
Posted: January 26th, 2012, 5:04am EST
Job Category Production/Manufacturing/Engineering
Location Bangalore
Experience 1.00 to 6.00 years
Education B.Sc, B.Tech, M.Sc
Skills
Good knowledge on centrifuge, reactors, filters pumps, techniques & aseptic operations. • Should have experience in Current Good manufacturing practices. (cGMP) • Good documentation skills • Awareness of audit requirements... -
Manager - Formulation Development
Posted: January 26th, 2012, 4:45am EST
Job Category Medical/Healthcare/Bio-Tech
Location Bangalore
Experience 6.00 to 10.00 years
Education M.Pharm, PHD
Industry Type Marketing
Job Description
oversee the outsourced formulation development of Generic formulations
Rev... -
Manager - International Marketing
Posted: January 26th, 2012, 4:43am EST
Job Category Maketing and Communications
Experience 5.00 to 7.00 years
Education M.B.A
Job Description
To work in the European Operation team at the Head Office .
To develop new business relations and managing existing ones.
To have P&L responsibilities for the territory / region.
Strategizing growth of the territory / r... -
Duty Doctor at Biocon
Posted: January 26th, 2012, 4:40am EST
Location Bangalore
Experience 0.00 to 0.00 years
Education MBBS
Job Description
Screening and maintaining complete data of volunteers
General physical examination of volunteer
To review biochemistry, hematology, serology, urine test results, ECG, Chest X Ray and any other lab test values,
Co-ordinate with Central... -
ASSOCIATE MANAGER - Production
Posted: January 26th, 2012, 4:38am EST
Job Category Production/Manufacturing/Engineering
Location Bangalore
Experience 8.00 to 15.00 years
Education M.Sc, M.Tech
Job Description
Planning, organizing, staffing and maintaining plant operations.
Scheduling activities and plan resources in order to meet deadlines.
Scale up of n... -
Associate Scientific Manager
Posted: January 26th, 2012, 4:34am EST
Job Category Medical/Healthcare/Bio-Tech
Location Bangalore
Experience 2.00 to 4.00 years
Education MBBS, MD/MS
Job Description
Define TPP and draft CDP for the assigned projects, update the same and keep updated based on inputs from various line functions
Medical m... -
CLINICAL Project Manager
Posted: January 26th, 2012, 4:31am EST
Job Category Medical/Healthcare/Bio-Tech
Location Bangalore
Experience 4.00 to 10.00 years
Education M.Pharm, M.Sc, Ph.D, PHD
Job Description
Lead the global matrix CTT to ensure all trial deliverables are met according to timelines, budget, quality standards & procedu... -
Manager- IT
Posted: January 26th, 2012, 4:28am EST
Job Category IT-Software
Location Bangalore
Experience 6.00 to 10.00 years
Education B.E, B.Tech, M.C.A
Industry Type IT Software
Job Description
Working on a full life-cycle implementations of Dynamics AX solutions
&nbs... -
Manager at Bangalore
Posted: January 26th, 2012, 4:23am EST
Job Category Medical/Healthcare/Bio-Tech
Location Bangalore
Experience 9.00 to 12.00 years
Education MPHARM, MS II
Skills
Strong knowledge and experience in handling regulatory requirements, functions with Biologicals/API/Sold Dosage particularly with filing with USFDA and EU for the fin... -
Manager at Biocon
Posted: January 26th, 2012, 4:18am EST
Job Category Maketing and Communications
Location Bangalore
Experience 4.00 to 8.00 years
Education M.B.A
Job Description
To spearhead companies API - Business Development for Indian Clients
Key Account Management for major clients with special focus on Regulated Market Projec... -
Question on Schaffer Collateral
Posted: January 26th, 2012, 1:15am EST
Hi,
I'm new to electrophysiology techniques as I did my undergrad in Human genetics. Right now I'm trining in 'in vivo' electrophysiology techniques. Lowering a bipolar stimulation electrode and a recording electrode into the hippocampus to induce LTP by using three trains of HFS at 200 hrz within 5 minute intervals at the Schaffer Collateral.
I'm getting my response at a depth of approx 2.8-3.1 mm down depending on the rat which are 310-400 g in weight. However even though I am ... -
Freezing and Thawing cancer cells
Posted: January 26th, 2012, 1:00am EST
I have been freezing cells in a freezing medium consisting 10% DMSO, 20% FBS and 70% RPMI or DMEM. The cells will be stored overnight at -80oC and then transfered to liquid N2 . We make our own freezing medium as indicated above. The problem comes when we want to use these cells after storage. All the cells are dead and there are no viable ones despite storage at the appropriate density and having been stored at greater than 90% viability. W... -
looking for water soluble film forming polymers after drying of water
Posted: January 25th, 2012, 8:50pm EST
Hi,
I am looking for water soluble polymers which form film after drying of water from their water dispersion such as polyvinylpyrrilidone. could you please suggest me some more polymers?
Thanks. I am looking forward to your suggestions.
Regards, -
Problem in repeatability of Real time PCR results
Posted: January 25th, 2012, 8:33pm EST
Dear Friends,
We are working on HBV quantification by using Rotor gene with Artus HBV PCR kit, the problem here is we are trying to work in 1/4 of the recommended volume and it is not showing similar results with the full volume results.
We have taken 7.5 microliters of master mix and 5 microliter of Extracted DNA and the recommended volume is 30 microliter of master mix and 20 microliter of extracted DNA
The results shows as follows.
For 12.5 microliter volume r... -
Adipose derived stem cells
Posted: January 25th, 2012, 4:27pm EST
Does anyone know what is the diameter of adipose derived stem cells?
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Your submissions to Journal of Errology @journalerrology
Posted: January 19th, 2012, 5:10pm EST
Dear Researchers,
Journal of Errology (JoE) is an experimental online research repository that enables sharing and discussions on those unpublished futile hypothesis, errors, iterations, negative results, false starts and other original stumbles that are part of a larger successful research in biological sciences.
We are open for submissions.. Make sure you recycle your experiences...
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High Hg levels in blood
Posted: January 19th, 2012, 10:03am EST
I am using Agilent 7700x (He collision mode) for Hg quantification in blood.
No memory effects we have.Calibration curve is linear,recovery is correct, QC and background are good.
But when we read "normal" samples (expected concentration below 5 ppb) ,in some samples , (20-30%) ,we found values about 10-15 ppb. Wht?s hapenning? .
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MDCK morphology
Posted: January 19th, 2012, 5:57am EST
Hi there,
I'm new to the whole virus studying world and I'm working on a line of cells that are new to me, MDCK cells. Is it true that if they become over confluent they start to transform?
I've been having a hard time finding images of transformed vs non-transformed MDCK cells.
The post-doc in my lab has told me that my cells have changed (and I've thrown away and regrew cells twice already) but they still look similar to when I first seeded them (in my opinion), the... -
PhD Student in Molecular Mechanisms of Neurodegenerative Diseases
Posted: January 19th, 2012, 4:53am EST
Description:
Within the newly established C.H.S. research group Proteostasis in Neurodegenerative Disease at CellNetworks and DKFZ we offer a
3-year PhD position in molecular mechanisms of neurodegenerative disease.
The recently established C.H.S. research group "Proteostasis in Neurodegenerative Disease" at the German Cancer Research Center (DKFZ) and CellNetworks is seeking a PhD student interested in mechanisms of protein misfolding and i... -
PostDoc in Neurodegenerative Disease Mechanisms
Posted: January 19th, 2012, 4:50am EST
Description:
Within the newly established C.H.S. research group Proteostasis in Neurodegenerative Disease at CellNetworks and DKFZ we offer a
Postdoc position in molecular mechanisms of neurodegenerative disease.
The recently established C.H.S. research group "Proteostasis in Neurodegenerative Disease" at the German Cancer Research Center (DKFZ) and CellNetworks is seeking a motivated PostDoc interested in mechanisms of protein misfolding and its link to ... -
Bovine Insulin Substitution in Cell Culture
Posted: January 19th, 2012, 4:20am EST
Good Afternoon,
I am hoping to start culturing a new human prostate cell line (MDA-PCa-2B) that is a bit troublesome in culture. ATCC suggests making an alternative media that contains a laundry list of items including bovine insulin. I am currently having a hard time getting bovine insulin from our local supplies and was wondeirng if anyone knows of a good substitution. Can i substitute insulin from another species? -
Manager - Formulation Development
Posted: January 19th, 2012, 3:27am EST
Job Category Medical/Healthcare/Bio-Tech
Location Bangalore
Experience 6.00 to 10.00 years
Education M.Pharm, PHD
Industry Type Marketing
... -
Executive in Biocon
Posted: January 19th, 2012, 3:22am EST
Job Category Quality Assurance
Location Bangalore
Experience 0.00 to 3.00 years
Education MSC(HONS)
Job Description
· To collect samples and to carry ou... -
hydrophobic protein
Posted: January 19th, 2012, 3:18am EST
Hallo! I need to purify a very hydrophobic, transmembrane protein (30-40 kDA). It is actually one in a family of related proteins. The goal would be to be able to separate all of them, but I would start with one.
In the literature I found people separating one of these proteins with PRP-3 from Hamilton (300 A I think ). In the Hamilton web site I found PRP-3 columns 10 or 12-20 uM. Which one would be more suitable to my study? WEhant about the column lenght?
Thanks! -
Manager - International Marketing in Biocon
Posted: January 19th, 2012, 2:50am EST
Job Category Maketing and Communications
Experience 5.00 to 7.00 years
Education M.B.A
Job Description
To work in the European Operation team at the Head Office .
To develop new business relations and managing existing ones.
To have P&L responsibilities for the territory / region.
Strategizing growth of the territory / region
Business development & Sale... -
JR.EXECUTIVE in Biocon
Posted: January 19th, 2012, 2:46am EST
Job Category Production/Manufacturing/Engineering
Location Bangalore
Experience 0.00 to 2.00 years
Education B.Sc
Job Descriptio... -
ASSOCIATE MANAGER - Production
Posted: January 19th, 2012, 2:43am EST
Job Category Production/Manufacturing/Engineering
Location Bangalore
Experience 8.00 to 15.00 years
Education M.Sc, M.Tech
Job Description
· ... -
Jr. EXECUTIVE in Biocon
Posted: January 19th, 2012, 2:34am EST
Job Category Production/Manufacturing/Engineering
Location NA
Experience 0.00 to 2.00 years
Education BSC(HONS), Diploma
Skills NA
... -
Scientific Manager -Analytical Development
Posted: January 19th, 2012, 2:30am EST
Job Category Medical/Healthcare/Bio-Tech
Location Bangalore
Experience 5.00 to 12.00 years
Education MSc, PHD
Skills and  ... -
Manager - Formulation Development at bangalore
Posted: January 19th, 2012, 2:25am EST
Job Category Medical/Healthcare/Bio-Tech
Location Bangalore
Experience 6.00 to 10.00 years
Education M.Pharm, PHD
Industry Type Marketing
Job Description ... -
CLINICAL Project Manager at bangalore
Posted: January 19th, 2012, 2:21am EST
Job Category Medical/Healthcare/Bio-Tech
Location Bangalore
Experience 4.00 to 10.00 years
Education M.Pharm, M.Sc, Ph.D, PHD
Skills NA
Industry Type &nbs... -
Executive/Jr Executive in Biocon
Posted: January 19th, 2012, 2:14am EST
Job Category Medical/Healthcare/Bio-Tech
Experience 1.00 to 3.00 years
Education B.Sc
Skills good at chemistry and to understand the basic biological terms
Job Description
... -
Mutation General qus.
Posted: January 19th, 2012, 1:59am EST
Hello all as the title of this post might show
i am woundering on how to knock down a gene that code for (bacterial protein) ?
how you decied that ? and short explanation of the procedure for each method with advantages and disadvantages ???
thanks guys and any replay is appreciated
cheers... -
Transduction troubleshooting: low efficiency
Posted: January 18th, 2012, 9:31am EST
Hello,
I am currently trying to get good transfection efficiency after infection of the bone marrow cells with the ecotropic murine stem cell retrovirus (MSCV). In brief, I have transfected my ecotropic virus (MSCV) into 293T cells and tested for GFP expression after growing then in NIH3T3 cells. Luckily, I got 90-95 % positive for GFP, analyzed by FACS. However, when the same virus is transduced into primary bone marrow cells by spinfection at 2000 rpm for 1.5 h, I am only getting ... -
DAC Value
Posted: January 18th, 2012, 7:57am EST
Is it normal for the DAC value to be:
Be 6.8
Co 8.5
In 8.5
and still pass the SmartTune Autolens? I was assuming that the DAC values had to go up linearly. Is this something that would affect Mn not passing a calibration curve????
Thanks in advance. -
Muscular dystrophy podcasts
Posted: January 12th, 2012, 7:33am EST
Muscular dystrophy podcasts from Nationwide Children's (Columbus, Ohio) and the Wellstone Center.
http://www.nationwidechildrens.org/muscular-dystrophy-podcast
Steve Wilton's podcast is on exon-skipping and Morpholinos.
http://www.nationwidechildrens.org/Document/Get/97953 -
Post doc in computational RNA systems biology
Posted: January 12th, 2012, 5:38am EST
Center for non-coding RNA in Technology and Health (RTH) at Department of Basic Animal and Veterinary Sciences, University of Copenhagen invites applications for a position as post doc for 2 years and 3 months (with a potential extension for additional 3 months) from March 1st, 2012, or as soon as possible thereafter.
Job description
The outset of the post doc project is to correlate the ncRNA candidates to protein coding genes through RNA-RNA and RNA-protein interactions a... -
PhD Position at CIC bioGUNE in Spain
Posted: January 12th, 2012, 5:13am EST
PhD Position
(Bilbao, Spain)
4-year FPI contract associated to MCINN Grant CTQ2011-28680
Topic: Recognition of chromatin structure by the ING tumor suppressors
Where: At CIC bioGUNE, near Bilbao, Spain, under the supervision of Dr. Francisco J. Blanco, Group leader in the Structural Biology Unit
Who: A motivated graduate student with interest in structural biology.
When: The contract will commence in summer 2012. By then the student shoul... -
More Bioscience Animation
Posted: January 12th, 2012, 5:13am EST
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Principal Investigator Vacancies at CIC bioGUNE
Posted: January 12th, 2012, 4:58am EST
Principal Investigator Vacancies
(Bilbao, Spain)
The Center for Cooperative Research in Biosciences (CIC bioGUNE) is a non-profit organization created to promote scientific research and technological innovation at the highest level in the Basque Country. Established by the Department of Industry, Technology and Innovation of the Government of the Autonomous Community in the Basque Country, CIC bioGUNE conducts research in the areas of Metabolomics, Proteomics, Genomics, Cellular an... -
PhD Position in spain (Bilbao)
Posted: January 12th, 2012, 4:52am EST
PhD Position
(Bilbao, Spain)
4-year- FPI contract associated to MICINN Grant SAF2011-29851.
Topic: Role of S-adenosylmethionine in hepatic carcinogenesis.
Where: At CIC bioGUNE, near Bilbao, Spain, under the suprvision of Dr. José M Mato, Group leader in the Metabolomics Unit.
When: The contract will commence in summer 2012. By then, the student should hold a Master degree to have access to the PhD program of a Spanish University.
... -
Post-doctoral Position in Spain
Posted: January 12th, 2012, 4:43am EST
Post-doctoral Position
(Bilbao, Spain)
The position is available to work in an ongoing European collaborative project to develop nanoparticle/siRNA-based technology as a therapeutic approach for cancer (NANOTHER) in the laboratory headed by Dr Edurne Berra at CIC bioGUNE, a young and growing research centre of excellent facilities.
The position requires a highly motivated Ph.D. with a strong experience in biochemistry, molecular and cell biology. Background in animal han... -
Platform Specialist in Bioinformatics (Derio, Spain)
Posted: January 12th, 2012, 4:34am EST
Platform Specialist in Bioinformatics
(Derio, Spain)
A Platform Specialist in Bioinformatics position is available at the Genome Analysis Platform of CIC bioGUNE. The main lines in the platform involve the set up and development of tools for high-throughput genotyping, gene expression and methylation characterization by means of arrays and/or sequencing data acquisition (mRNA-Seq, microRNAs-Seq, targeted DNA regions resequencing, ChIP-Seq, etc).
The research facilities a... -
Postdoctoral position
Posted: January 12th, 2012, 4:23am EST
Postdoctoral position
(Bilbao, Spain)
The possibility to apply a three-year postdoctoral fellowship at the call of the Asociación
Española contra el Cancer (AECC) is available.
Aid for cancer researchers modality A 2012
• Status: open
• Start date: 2011/11/15
• Description: Aid to doctors or post residents to carry out a research project in a
Spanish center.
In the laboratory of Dr. Maria Luz Martinez Chanta,... -
Postdoctoral Research Fellow
Posted: January 12th, 2012, 4:20am EST
Postdoctoral Research Fellow
(Bilbao, Spain)
Applications are invited for a Postdoctoral Research Fellow to join a group led by Robert Kypta in the
Cell Biology and Stem Cells Unit, which works in the general area of Wnt and GSK-3 signalling. The
project will focus on the roles of GSK-3 isoforms in differentiation of stem cells and in cancer cells.
The position is available for a period of up to 36 months.
Applicants should have expertise in molecular an... -
IEF gels for blotting
Posted: January 12th, 2012, 1:58am EST
Hi,
Anyone who has run an IEF-gel and then performed western blot? I've looked at the NuPAGE IEF-gels, but can't find anything regarding compatibility with western blot. Are there any other systems worth testing?
Thanks!
-
Zinc Contamination
Posted: January 11th, 2012, 11:44pm EST
I was wondring if anyone has had a zinc contamination in either their lab or in sample prep. How did you handle it and where was it coming from? I am currently using the ELAN DRCe.
Thanks,
T
-
gene-break transposons, zfishbook
Posted: January 11th, 2012, 9:58am EST
This is gene engineering at the edge: "implementing gene-break transposons to create expression-tagged, revertible mutations in zebrafish genes". http://zfishbook.org/
Here's a description of the technology:
Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC. In vivo protein trapping produces a functional... -
Postdoctoral researcher, Circulation in the South West Indian Ocean
Posted: January 11th, 2012, 7:33am EST
Postdoctoral researcher (3 yrs)
Circulation in the South West Indian Ocean
Vacancy number 2011-063
Project description:
This project aims at determination of the circulation through the Mozambique Channel and in the East Madagascar Current, the long-term temporal and spatial structure, and its hydrographical properties. Our observations near Madagascar already started in 2000 and are still ongoing. The project is jointly funded by the Netherlands Organization for Scie... -
PhD Students - The impact of biological invasions on the food web of the Wa
Posted: January 11th, 2012, 7:21am EST
NIOZ Royal Netherlands Institute for Sea Research
In the Frame of the bilateral Dutch-German BMBF/NWO Project: The impact of biological invasions on the food web of the Wadden Sea (INFOWEB) 2 open PhD vacancies have to be filled.
The main issue is to construct food web models for selected tidal basins and to consider the impact of invasive species on them. This task includes the processing of available long term and monitoring data for dominant species of the partic... -
SCIENTIST - Toxicogenomics
Posted: January 11th, 2012, 7:12am EST
SCIENTIST - Toxicogenomics
You are highly motivated and are always committed to deliver work of the highest quality. You encourage collaboration and enjoy working in a team. You are a passionate individual who likes creative and international work environments.
THE OPPORTUNITY
The successful candidate will join the Biological Systems Research Team at Philip Morris Products S.A. in Neuchâtel, Switzerland. He/she will play a major role... -
Scientist - Computational proteomics
Posted: January 11th, 2012, 7:09am EST
Scientist - Computational proteomics
YOU are highly motivated and always committed to deliver the best quality. You encourage collaboration and enjoy working in a team. You are passionate individual who likes creative and international work environments.
THE OPPORTUNITY
The successful candidate will join the Biological Systems Research Unit of Philip Morris Products S.A. in Neuchâtel, Switzerland and play a major role in developing and managing our computatio... -
Lead Laboratory Technician - Gene Expression (Affymetrix microarrays)
Posted: January 11th, 2012, 7:07am EST
Lead Laboratory Technician - Gene Expression (Affymetrix microarrays)
YOU are highly motivated and always committed to deliver work of the highest quality. You encourage collaboration and enjoy working in a team. You are a passionate individual who likes creative and international work environments.
THE OPPORTUNITY
The successful candidate will join the Biological Systems Research Unit of Philip Morris Products S.A. in Neuchâtel, Switzerland and w... -
Associate Staff scientist - Disease Research
Posted: January 11th, 2012, 7:04am EST
THE OPPORTUNITY
The successful candidate will join the Biological Systems Research Team at Philip Morris Products S.A. in Neuchâtel, Switzerland and will play a major role in the development and application of primary cell cultures to Translational Systems Biology and eventually to Product Testing. These approaches are focused on understanding more about the mechanisms of diseases caused by cigarette smoke, in order to support the development and assessment of new produ... -
Cell Signaling Scientist
Posted: January 11th, 2012, 7:02am EST
Cell Signaling Scientist
YOU are highly motivated and are always committed to deliver work of the highest quality. You encourage collaboration and enjoy working in a team. You are a passionate individual who likes creative and international work environments.
THE OPPORTUNITY
The successful candidate will join the Biological Systems Research Team at Philip Morris Products S.A. in Neuchâtel, Switzerland and will play a major role in the interpretation ... -
Product Development Engineer : Cork, Ireland
Posted: January 6th, 2012, 6:56am EST
University College Cork, Tyndall National Institute invites applications for an appointment as Product Development Engineer to develop and implement a Linux/Android based 802.11 WiFi product as part of an Industry funded project with a multinational commercial partner.
Tyndall National Institute (Tyndall), www.tyndall.ie, is Ireland’s largest research centre, specialising in defined areas of Information and Communications Technology (ICT).
Within Tyndall, the WSN tea... -
Research Associate-Loyola University Chicago
Posted: January 6th, 2012, 6:48am EST
Currently there is one job opening for a Research Associate available in an NIH-funded research group studying cardiovascular cellular physiology.
This postdoctoral position provides a unique training opportunity for a motivated individual who desires to work in a highly interactive environment conducting cutting edge research using state-of-the-art biophysical techniques, high-resolution imagin... -
Research Officer : Bath, United Kingd
Posted: January 6th, 2012, 6:33am EST
Research Officer (3 years fixed term contract) (BBSRC)
Pharmacy & Pharmacology
Salary: Starting from £29,972, rising to £35,788
Reference: JK871
Title: Paracrine regulation of endothelial cell motility and angiogenesis by deoxyribose-1-phosphate.
For information please email: G.Pula@bath.ac.uk, however please ensure that your application is
Deadline: 01 February 2012
For more details
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PhD Program Cardiovascular Research (TransCard) : Berlin, Germany
Posted: January 6th, 2012, 6:20am EST
The Helmholtz International Research School TransCard offers:
Fully-funded PhD positions in Translational Cardiovascular and Metabolic Medicine
Deadline: March 20th, 2012
The TransCard program is an attractive PhD program for several good reasons:
Excellent and challenging research projects ensured by high quality faculty.
Constant mentoring of Ph.D. progress through the implemented Ph.D. Committees.
A core lecture series to provide necessary background on... -
Research Associate- Bioinformatics
Posted: January 6th, 2012, 6:07am EST
Experience: 0 – 2 Year(s) Experience
Location: Rapture Biotech, 66 Dev Nagar Khandari Bypass, Agra, Uttar Pradesh – 282005
Qualification : M.Sc. or PG Diploma in Bioinformatics or Computational Biology/Masters of science / Bachelor of Technology
Deadline: Contact employer
For more details
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Biotech Faculty Job-NORTH ORISSA UNIVERSITY
Posted: January 6th, 2012, 6:00am EST
North Orissa University invites applications in the prescribed form from the eligible candidates for appointment to the posts of Professors, Readers and Lecturers.
Professor
Biotechnology – 1 Post
Reader
Biotechnology – 2 Posts
Lecturer
Biotechnology – 3 Posts
Deadline: 16.01.2012
For more details
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Faculty Job-Manipur University
Posted: January 6th, 2012, 5:49am EST
Applications are invited from Indian Nationals for filling up the following posts :
Professor/Associate Professor :
Biochemistry – 1
Biotech – 3
Life Sciences – 5
Application Fee : Rs.300/- (Rs.150/- for SC/ST/PWD) in the form of Bank Draft of SBI drawn in favour of Registrar, Manipur University, Canchipur payable at SBI, MU, Canchipur.
How to Apply : Application in the prescribed format should be send to the Registrar, ... -
Scientist Positions- RGCB
Posted: January 6th, 2012, 5:42am EST
Rajive Gandhi Centre for Biotechnology invites application for Scientific positons in Disease biology and Plant biotechnology
Deadline: April 30, 2012
For more details
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Sales Marketing Manager,Sales Marketing Manager-Biotech Desk Pvt. Ltd.
Posted: January 6th, 2012, 5:31am EST
Biotech Desk Pvt. Ltd. offers career opportunities in biotechnology and related fields.
Currently recruiting for Sales & Marketing Manager, to be stationed at (i) Mumbai/Pune (ii) Hyderabad (iii) Bangalore/Chennai.
Qualifications: Masters Degree in LifeSciences or related field. Prior experience in sales would be preferred.
Job profile involves:
Direct selling to researchers in Govt., sem... -
Assistant Professor - Research
Posted: January 6th, 2012, 5:16am EST
The University of Cincinnati is currently accepting applications for an Assistant Professor – Research. The researcher is expected to participate in the ongoing research projects in vision science using mesenchymal stem cell transplantation to treat congenital and acquired corneal diseases. The researcher will work towards independent research projects and support.
Job Description: On a full-time basis Research Faculty Member's primary responsibility is research, w... -
Senior Research Scientist 1- Cell Signaling in Promega
Posted: January 6th, 2012, 5:05am EST
Promega is looking for a candidate with a PhD in biochemistry, cellular, or molecular biology. A qualified candidate will have research experience using chemical biology approaches to develop research tools for interrogating cell signaling pathways.
An excellent record of productivity and interest to advance our product line in cell signaling research tools is necessary. The candidate should also have the desire to develop independent projects.
Minimum Requirements:
... -
Graduate, Postdoctoral and Senior Research Awards
Posted: January 6th, 2012, 4:58am EST
The National Research Council of the National Academies is accepting applications for awards for independent graduate, postdoctoral and senior research to be conducted in residence at US federal laboratories and affiliated institutions.
Awards include competitive stipends, relocation, professional travel and health insurance. Detailed information, including instructions for submitting online applications are at www.nationalacademies.org/rap Applicants should contact prospective... -
Research Associateship Programs for PhD
Posted: January 6th, 2012, 4:49am EST
The mission of the NRC Research Associateship Programs (RAP) is to promote excellence in scientific and technological research conducted by the U. S. government through the administration of programs offering graduate, postdoctoral, and senior level research opportunities at sponsoring federal laboratories and affiliated institutions.
In these programs, prospective applicants select a research project or projects from among the large group of opportunities listed on this website. ... -
Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling
Posted: January 6th, 2012, 3:48am EST
Lee HS, Sokol SY, Moody SA, Daar IO. Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling. Methods Mol Biol. 2012;839:91-104. -
Troubleshooting Bone marrow transduction
Posted: January 5th, 2012, 6:32am EST
Hello,
I am currently trying to get good transfection efficiency after infection of the bone marrow cells with the ecotropic murine stem cell retrovirus (MSCV). In brief, I have transfected my ecotropic virus (MSCV) into 293T cells and tested for GFP expression after growing then in NIH3T3 cells. Luckily, I got 90-95 % positive for GFP, analyzed by FACS. However, when the same virus is transduced into primary bone marrow cells by spinfection at 2000 rpm for 1.5 h, I am only ge... -
Hematopoietic stem cells, hematopoiesis and disease (zebrafish)
Posted: January 5th, 2012, 4:55am EST
Martin CS, Moriyama A, Zon LI. Hematopoietic stem cells, hematopoiesis and disease: lessons from the zebrafish model. Genome Med. 2011 Dec 29;3(1):83. [Epub ahead of print]
Review
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Doses/concentration of plant crude extract use to treat cell
Posted: January 5th, 2012, 3:10am EST
I am doing cell culture & i am wondering what doses/ concentration (plant crude extract is my sample) should i use to treat my cell in FRAP test, an in-vitro antioxidant test....i get to know that i can follow the standard doses form OECD..But i fail to find it, most of the OECD are for in vivo test..Any other standard protocal i can follow to determine the doses for treating my cell?
Thx!! -
International PhD Program in Microbiology at Germany
Posted: January 4th, 2012, 10:56pm EST
International PhD Program in Microbiology at International Max Planck Research School, Germany
Eligibility:
The programme is open to students from Germany as well as students from abroad. Applicants must hold a Master of Science (MSc) degree, or equivalent, in biology, biochemistry, biogeochemistry, genetics, molecular biology, microbiology, bioinformatics, chemistry, or related fields. The degree has to be conferred before the programme starts.
Scholarship Descriptio... -
Call for Applications at Boysen-Tu Dresden Graduate College, Germany
Posted: January 4th, 2012, 10:53pm EST
Call for Applications for Graduate Students at Boysen-Tu Dresden Graduate College, Germany
Eligibility:
Applicants must have a better than average university degree in the field of their research, fulfill the requirements of the respective regulations for dissertations in the faculty in which they seek their doctoral degree, and show a strong motivation for interdisciplinary research and teamwork.
Their application should contain a CV, copies of documents of their previo... -
PhD Stipends for Research on Global Biogeochemical Cycles at IMPRS, Germany
Posted: January 4th, 2012, 10:48pm EST
PhD Stipends for Research on Global Biogeochemical Cycles at IMPRS, Germany
Eligibility:
Applications for the program are open to well-motivated and highly-qualified students from all countries. A prerequisite is a diploma or master of science degree in geophysical sciences, environmental sciences, biological sciences, physics, chemistry, computer sciences or related fields, including a corresponding thesis. Proficiency in English is required since English is the official language ...