Hello,
I am currently trying to get good transfection efficiency after infection of the bone marrow cells with the ecotropic murine stem cell retrovirus (MSCV). In brief, I have transfected my ecotropic virus (MSCV) into 293T cells and tested for GFP expression after growing then in NIH3T3 cells. Luckily, I got 90-95 % positive for GFP, analyzed by FACS. However, when the same virus is transduced into primary bone marrow cells by spinfection at 2000 rpm for 1.5 h, I am only getting ...
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Transduction troubleshooting: low efficiency
Posted: January 18th, 2012, 9:31am EST