Hallo! I need to purify a very hydrophobic, transmembrane protein (30-40 kDA). It is actually one in a family of related proteins. The goal would be to be able to separate all of them, but I would start with one.
In the literature I found people separating one of these proteins with PRP-3 from Hamilton (300 A I think ). In the Hamilton web site I found PRP-3 columns 10 or 12-20 uM. Which one would be more suitable to my study? WEhant about the column lenght?
Thanks!
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hydrophobic protein
Posted: January 19th, 2012, 3:18am EST