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Latest discussion from ScientistSolutions.com

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  Problem in repeatability of Real time PCR results

  Posted: January 25th, 2012, 8:33pm EST

  Dear Friends,
  We are working on HBV quantification by using Rotor gene with Artus HBV PCR kit, the problem here is we are trying to work in 1/4 of the recommended volume and it is not showing similar results with the full volume results.
  We have taken 7.5 microliters of master mix and 5 microliter of Extracted DNA and the recommended volume is 30 microliter of master mix and 20 microliter of extracted DNA
  The results shows as follows.
  For 12.5 microliter volume r...