Feeds
5681 items (5681 unread) in 8 feeds
-
Wisconsin Green Party - (16 unread)
-
Green Party RSS Feed (524 unread)
-
Global Warming Effects, Global warming facts, Causes of global warming, Stop Global Warming, Global Warming Awareness
(12 unread)
-
Latest discussion from ScientistSolutions.com (4301 unread)
-
Science Blog From Networlddirectory
(627 unread)
-
Seed Magazine: News
(15 unread)
-
Green Electricity
(105 unread)
-
Cynthia McKinney's Facebook Notes (81 unread)
Latest discussion from ScientistSolutions.com
-
Freezing and Thawing cancer cells
Posted: January 26th, 2012, 1:00am EST
I have been freezing cells in a freezing medium consisting 10% DMSO, 20% FBS and 70% RPMI or DMEM. The cells will be stored overnight at -80oC and then transfered to liquid N2 . We make our own freezing medium as indicated above. The problem comes when we want to use these cells after storage. All the cells are dead and there are no viable ones despite storage at the appropriate density and having been stored at greater than 90% viability. W...